The Indiana Southern Railroad (reporting mark ISRR) is a short line or Class III railroadoperating in the United States state of Indiana. It began operations in 1992, and was acquired byRailAmerica in 2000. RailAmerica was itself acquired by Genesee & Wyoming in December of 2012. 
Indiana Southern Railroad operates 176 miles of track from Indianapolis to Evansville. From Mars Hill (a neighborhood on the southwest side of Indianapolis) southwest through Martinsvilleand Spencer to Bee Hunter in Greene County, the ISRR runs on tracks that once made up the majority of the former Indianapolis & Vincennes Branch of the Pennsylvania Railroad.State Route 67 parallels the ISRR along much of this section.
The railroad's traffic comes mainly from coal and grain products, including corn and soybeans. The ISRR hauled around 70,000 carloads in 2008.It also is supplying coal to the new Edwardsport power plant starting in 2012. There will be around 2 trains per week to supply it.